• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    1532848 Purifying proteins P G S LINDROOS 19 Nov 1976 [11 Dec 1975] 48376/76 Heading C3H A method for separating iron compounds from the protein in a hemoglobin solution, comprises adding to said solution an organic solvent comprising ethanol in an amount such that the total ethanol content in the solution is at least 40% by volume, adjusting the pH of the liquid to a value lower than 4À5, whereby iron compounds in the solution are agglomerated, and thereafter separating the agglomerated iron compounds from the solution. The solution may be adjusted to a pH value which is less than 4À5 but greater than 2À5. Preferably the pH-value of the solution after agglomeration of the iron compounds is, before or after their separation, raised and wherein any fraction of blood protein precipitated thereby, with iron compounds included therein or adsorbed thereonto, is separated from the mixture. Before or after the agglomeration of iron compounds a minor quantity of broth of blood cells may be added to the solution and any flaky material of fragments of cell membrane formed thereby, together with iron compounds adsorbed thereonto, is then separated from the mixture. The organic solvent preferably includes a minor proportion of glycerin or ethylene glycol or methanol or acetone or ethylacetate or propanol or isopropanol or butanol. The iron compounds may be separated from the solution by centrifuging or a cyclone process or filtering or ultrafiltering or sedimenting or adsorption on to voluminous or solid material, and the protein may be recovered from the solution by precipitation or adsorption on to solid or voluminous material or ultrafiltering or evaporation of the solution or drying or concentration of the solution by means of freezing. The ethanol content in the solution after the agglomeration of iron compounds may be increased before or after their isolation and the fraction of blood protein precipitated thereby, together with any iron compounds included therein or adsorbed thereonto, may then be separated from the mixture. Before addition of ethanol to the solution and lowering of the pH of the solution, the solution is preferably cooled to a temperature of between zero and -20‹ C. and wherein this temperature is maintained during the entire further treatment.
    公开号:SU1071208A3
    申请号:SU762427755
    申请日:1976-12-10
    公开日:1984-01-30
    发明作者:Йеран Сигвард Линдроос Пауль
    申请人:Пауль Йеран Сигвард Линдроос (Швеци )；
    IPC主号:
    专利说明:

    The invention relates to methods for separating hemoglobin derivatives of iron compounds from protein, mainly globin, from a liquid containing blood substances, which can subsequently be used in the food industry due to its nutritional value, or while maintaining its functional properties. as a structural agent. A laboratory method is known for separating liquids containing blood substances (iron compounds and globin by extraction, using solvents such as methyl ethyl ketone, acetone and dimethylformamide ClJ .... However, the product obtained by this method is unsuitable for or as a food product. The closest to the proposed method is the separation of liquids containing blood substances, cooling the hemoglobin solution, adding precipitate lint, filtering the mixture obtained and C23. However, the known method is rather complicated. The purpose of the invention is to simplify. The aim is achieved by the fact that when implementing the method of separation of a liquid containing blood substances by cooling the hemoglobin solution, adding precipitant, filtering the mixture obtained, the hemoglobin solution is cooled to temperatures of +8 -, ethanol is added to a concentration of 40-70%, the pH is adjusted to 2.54, 5, inorganic salts of sodium or potassium are added, the red corpuscular pulp is separated, the separated agglomerates are separated from the globin solution Zom. Example 1. Pulp of red blood cells from blood from the slaughterhouse, along with sodium citrate; as an anticoagulant agent, it is treated with ethanol and water in such a way as to cause rupture of cell membranes. Cell membrane fragments, v, are removed by centrifugation. A hemoglobin solution is obtained containing 15% dry matter, mainly hemoglobin, 33% by volume of ethanol, and the rest is water. The pH value is 5.3, the color is dark red .. 3 g of this hemoglobin solution is cooled to a temperature in a beaker, and the solution consisting of is dripped into the solution with continuous stirring and cooling. 0.5 ml of 1M hydrochloric acid and from 10 ml of 96% ethanol at -12 ° C. After adding the solution, the temperature is co; is -12 ° C, and the pH value is 3.1. The ethanol content in the mixture is 78% by volume. The pH is determined so that the samples of the mixture are diluted with three times the volume of water before determination. The mixture, which has a black-brown color, is centrifuged at 27,000 volts for 10 minutes in a Sorvait centrifuge at a temperature and a black paste in an amount of 1.5 GW, a solids content of 2.9%, and a light brown liquid on the surface layer . A light gray precipitate is precipitated from the liquid of the surface layer by adding water to 50% and bringing the pH to 7.5. The temperature is maintained at -7 ° C. The precipitate is removed by centrifugation and a gray paste is obtained in the amount of 0.95 g with a solids content of 28%. From the results it follows that sulfur paste consists of a protein with an iron content of about 5% of the total iron content in hemoglobin. Example 2. A solution of hemoglobin solution (with characteristics as in example 1) at -7 ° C is added dropwise with continuous stirring and cooling to a beaker with a solution of 0.45 ml of 1M hydrochloric acid, 7.5 ml of water and 10 ml of 96% ethanol at. After the addition, the temperature melts and the pH value is 3.3. The ethanol content in the mixture is adjusted to 50 vol.%. The mixture, which has a black-brown color, is centrifuged at 10,000 g for 10 minutes and a black paste with an amount of dry matter of 0.03 g and a light yellow liquid of the surface layer is obtained. The liquid of the surface layer is divided into two equal parts. From one part, a gray precipitate is precipitated at pH 7.5, which after separation by centrifugation and drying gives 0.12 g of dry matter. Another part is mixed with 5 ml of ethanol and 0.2 ml of sodium chloride in 10% aqueous solution with continuous stirring and cooling and subjected to ultrafiltration at a temperature of -6 ° C. In a cell of Amikon Diaflou (.Amicpn DiafBo) with XM 300 membrane A light yellow Color filtrate is obtained, with the membrane covered with a dark film. After dilution with water to a water content of 50% and at a pH of 7.5, a precipitate is precipitated from the filtrate, its color is from white to light gray, from which 0.11 g of dry matter is obtained after centrifugation and drying. Example 3. A solution of hemoglobin solution (with the same characteristics as in Example 1) at 4 ° C is added dropwise with continuous stirring and cooling in a beaker with a solution of 0.45 ml of glacial acetic acid, 0.8 ml of water and 5 ml of 96% ethanol at -4 ° C. . After the addition, the temperature was -4 ° C, and the pH value was 3.8. The ethanol content of the mixture is 62% by volume. The black-brown mixture is centrifuged at 27,000 g for 10 minutes to obtain a black paste with a dry matter content of 0.11 g and a light yellow liquid on the surface layer. After dilution with water to a water content of 50% and at a pH value of 7.5, a precipitate of light brown to gray color is precipitated from the surface layer from which, after centrifuging and drying, 0.25 g of dry matter is obtained. Example 4. A solution of hemoglobin solution (with the same characteristics as in example 1) is added dropwise with continuous stirring and cooling to a beaker with a solution of 0.45 ml of 1m hydrochloric acid, 0.2 ml of 20% - aqueous sodium chloride and 10 ml of 96% ethanol at -15 ° C. After the addition, the temperature is -10 ° C and the pH value is 3.3. The ethanol content of the mixture is 77% by volume. The black-brown mixture was centrifuged at 10,000 g for 10 minutes to obtain a black paste with a dry substance content of 0.09 and a surface layer liquid having a color from light yellow to light brown. After dilution with water to a water content of 50% at a pH value of 7.5, a gray precipitate is deposited from the surface layer, from which, after centrifugation and drying, .0.30 g dry matter is obtained. Example 5. 6 ml of 96% ethanol is added dropwise with continuous stirring and cooling to a beaker with 3 g of hemoglobin solution (with the same characteristics as in Example 1). The temperature is -10 ° WITH. Into this suspension of hemoglobin, aprons are dropped and continuously stirred and cooled with 0.4 ml of 1M hydrochloric acid in 3 ml of 96% ethanol. After adding the temperature is -10 ° C, and: pH value 3.4. The ethanol content of the mixture is 77% by volume. The black-brown mixture is centrifuged at 20,000 R10 min and a black paste is obtained with a dry substance content of 0.08 g and a light yellow liquid on the surface layer. , 0.19 ml of 0.5M sodium hydroxide in 10 ml of 96% ethanol is added dropwise with continuous mixing of the surface layer and cooling into liquid. After the addition, the temperature is -10 ° C and the pH value is 4.35. The ethanol content of the mixture is 85% by volume. The precipitate obtained, the blood protein fraction with adsorbed iron compounds is removed by centrifugation to obtain a black and red haze with a dry matter content of 0.08 g. From a very slightly yellowish-colored surface layer liquid after adding water to a water content of 50% and increasing the pH to 7, 5 with continuous cooling, a light gray precipitate is precipitated, from which, after separation by centrifugation and drying, 0.22 g of a dry broadcast are obtained. . Example 6: A solution of hemoglobin solution (with the same characteristics as in example 1) is added dropwise with continuous stirring and cooling to a beaker with a 0.45 ml solution. 1 m of hydrochloric acid and 10 ml of 96% ethanol at -8 ° C. After the addition, the temperature melts and the pH value is 3.3. The ethanol content of the mixture is 78 bb.%. Black-brown mixture centrifuged. at 100QO g for 10 min, resulting in a black paste with a dry matter content of 0.06 g and a light brown liquid on the surface layer. 1 ml of the suspension with 0.1 g of red blood cell pulp in equal volumes of ethanol and water at -8 ° C and at pH 5.3 is added dropwise with continuous stirring and cooling of the surface layer into the liquid. After the addition, the temperature is -8 ° C, and the ethanol content is 75% by volume. The mixture is centrifuged at 10,000 g for 10 minutes, resulting in a small amount of black paste and a superficial liquid having a color from light brown to light yellow. From the liquid of the surface layer, the light gray precipitate is precipitated in the same manner as in Example 5, from which, after separation by centrifugation and drying, 0.25 g of dry matter is obtained. Example 7. A solution of hemoglobin solution (with the same characteristics as in example 1) is added dropwise with continuous stirring and cooling to a beaker with a solution of 0.50 ml of 1m hydrochloric acid, 8 ml of 96%: ethanol and 2 ml of glycerin, at 8 ° C. After the addition, the temperature is -8 ° C and the pH value is 3.1. Complete co
    权利要求:
    Claims (1)
    [1]
    METHOD FOR SEPARATING A LIQUID CONTAINING BLOOD SUBSTANCE, by cooling a hemoglobin solution, to- adding a precipitant, filtering the resulting mixture, characterized in that, in order to simplify the process, the hemrhlobin solution is cooled to a temperature of 4-8- -20 ° C, ethanol is added to a concentration of 40-70%, the pH is adjusted to 2.5 -4.5, inorganic salts of sodium or potassium are added, pulp of red blood cells, the released agglomerates are separated from the globin solution in a known manner.
    1 1071208,
    类似技术:
    公开号 | 公开日 | 专利标题
    US4320050A|1982-03-16|Process for selectively extracting dyestuffs contained in cyanophyceae algae, the so-extracted dyestuffs and their use, particularly in foodstuffs
    US4098780A|1978-07-04|Method of treating liquids containing blood substances
    Crewther et al.1967|The preparation and properties of a helix-rich fraction obtained by partial proteolysis of low sulfur S-carboxymethylkerateine from wool
    Partridge et al.1958|The chemistry of connective tissues. 4. The presence of a non-collagenous protein in cartilage
    SU1071208A3|1984-01-30|Method for separating liquid containing blood substances
    Kane1970|Direct isolation of the hyaline layer protein released from the cortical granules of the sea urchin egg at fertilization
    Freer et al.1973|Effects of staphylococcal α-toxin on the structure of erythrocyte membranes: a biochemical and freeze-etching study
    Wolfe et al.1961|Migration of histones from the nuclei of isolated cerebral tissues kept in cold media
    SU1660573A3|1991-06-30|Method for heme concentrate preparation
    US2134256A|1938-10-25|Process of producing and refining organ extracts
    SU910135A3|1982-02-28|Process for separating hemo-iron from globine
    US3123539A|1964-03-03|Process for recovering catalase from
    Elgsaeter et al.1978|Animal carotenoids 15: Carotenoid distribution and carotenoprotein of Asterias rubens
    Brodersen et al.1963|Chloroform extraction of serum bilirubin in relation to its binding to proteins
    Freifelder1967|[70] The use of NaClO4 to isolate bacteriophage nucleic acids
    GB1430566A|1976-03-31|Isolation of proteins
    Hensarling et al.1974|Extraction of lipids from cottonseed tissue: IV. Use of hexane‐acetic acid
    WU et al.1971|Myosin stability in intact chicken muscle and a protein component released after aging
    US2460891A|1949-02-08|Separation of proteins from milk products
    US3086865A|1963-04-23|Clarification of beverages with animal blood albumin
    RU2007423C1|1994-02-15|Method of serum albumin purification
    SU1732806A3|1992-05-07|Method for separation hemoglobin to heme and globin
    Novikov et al.2001|Defatting and clarification of protein hydrolysates by using chitosan solutions
    SU732743A1|1980-05-05|Method for determining protein content of wine
    RU1824444C|1993-06-30|Process for preparing biological stimulant
    同族专利:
    公开号 | 公开日
    NZ182682A|1979-11-01|
    FI60104C|1981-12-10|
    FR2351604B2|1982-10-29|
    NL183218B|1988-04-05|
    AU509315B2|1980-05-08|
    NL183218C|1988-09-01|
    FR2351604A2|1977-12-16|
    FI753503A|1977-06-12|
    IT1121690B|1986-04-10|
    DE2656157A1|1977-06-23|
    NL7613665A|1977-06-14|
    IE49296B1|1985-09-18|
    AU2004176A|1978-06-01|
    CS200200B2|1980-08-29|
    GB1532848A|1978-11-22|
    DD127755A5|1977-10-12|
    FI60104B|1981-08-31|
    SE7513987L|1977-06-12|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    DE2423683C2|1974-05-15|1982-10-21|Bayer Ag, 5090 Leverkusen|0-Triazolyl phosphorus acid esters and ester amides, processes for their preparation and their use as insecticides, acaricides and nematicides|CA1126653A|1978-12-22|1982-06-29|Jan H. Luijerink|Process of preparing blood cell protein and hemefrom hemoglobin|
    SE440596B|1980-04-03|1985-08-12|Paul Goran Sigvard Lindroos|PROCEDURE FOR PREPARING A HOME CONCENTRATE FROM A MIXTURE OF HOME AND BLOOD SUBSTANCE RECOVERY BY DIVISION OF HEMOGLOBIN|
    DK144800C|1980-04-21|1982-10-25|Forenede Bryggerier As|PROCEDURE FOR THE EXTRACTION OF ENZYMES, PRIOR CU, ZN SUPEROXIDE DISMUTASE , CATALASE AND CARBONIC ACID ANHYDRASE, FROM BLOOD|
    IT1135153B|1981-01-23|1986-08-20|Consiglio Nazionale Ricerche|PROCESS FOR MAKING BLOOD INCOAGULABLE BY PROTEOLITHIC ENZYMES AND USE OF INCOAGULABLE BLOOD TO PRODUCE A PROTEIN CONCENTRATE FROM WHOLE BLOOD|
    FR2535173A1|1982-11-03|1984-05-04|Protein Sa|Products obtained from the blood of abattoir animals and method for obtaining them.|
    FR2548671B1|1983-07-07|1986-05-02|Merieux Inst|PROCESS FOR THE PREPARATION OF A GLOBIN FROM HEMOGLOBIN AND A GLOBIN OBTAINED BY THIS PROCESS|
    HU191320B|1985-03-20|1987-02-27|Horvath,Sandor,Hu|Oxidation process|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    SE7513987A|SE7513987L|1975-12-11|1975-12-11|WAY TO TREAT HEAVY SHOES CONTAINING BLOOD SUBSTANCES|
    [返回顶部]